鎮ㄥソ锛屾杩庢偍鏉ュ埌纭曞崥鍘熷垱璁烘枃缃戯紒
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宸村柗涓佹憾娑叉姂鍒舵柟鏍圭粨绾胯櫕浜岄緞骞艰櫕鐨勬渶浣虫祿搴?/h1>

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纭?a href="http://flywand.com/">鍗氳鏂囩綉鏄竴瀹朵笓涓氭彁渚涜鏂囧畾鍒朵慨鏀规湇鍔$殑缃戠珯锛屼笂10骞寸殑璁烘枃缁忛獙锛屾棤璁烘槸鏈璁烘枃銆佺澹鏂囪繕鏄湡鍒婅鏂囷紝鍗氬+璁烘枃锛屾垜浠兘鑳戒负鎮ㄦ彁渚涙柟渚裤€佸揩鎹枫€佸畨鍏ㄧ殑璁烘枃鏈嶅姟銆備互涓嬫槸纭曞崥璁烘枃缃戝皬缂栦负鎮ㄧ簿蹇冩寫閫夌殑涓€绡囩敓鐗╁璁烘枃姣曚笟璁烘枃鑼冩枃锛屽唴瀹规槸鍏充簬宸村柗涓佹憾娑叉姂鍒舵柟鏍圭粨绾胯櫕浜岄緞骞艰櫕鐨勬渶浣虫祿搴︺€傘€愮粨璁恒€戞鐗╂簮鐨勫姞宸村柗涓佹憾娑插湪1 mM娴撳害鏃跺J2鍦ㄤ綋澶栨湁鏄庢樉鐨勬姂鍒朵綔鐢?鏈夋垚涓烘柊鍨嬫潃绾垮墏鐨勫彲鑳姐€傘€備互渚涘弬鑰冦€備互渚涘悇浣嶅悓瀛﹀弬鑰冿紝甯屾湜瀵瑰ぇ瀹舵湁鎵€甯姪銆?/strong>
銆€鎽?nbsp;   瑕侊細銆€銆愮洰鐨勩€戞帰绌剁暘鑼勬牴绯诲垎娉岀墿涓姞宸村柗涓佹憾娑茶繍鐢ㄤ簬鐢熶骇鐨勬湁鏁堟祿搴︺€傘€愭柟娉曘€戦€氳繃LC/GC-MS鍒嗘瀽鐣寗鏍圭郴鍒嗘硨鐗╀腑鎴愬垎;閫氳繃瓒嬪寲鎬ц瘯楠屻€佹浜$巼璇曢獙鏉ユ帰绌朵笉鍚屾祿搴︾殑鍔犲反鍠蜂竵婧舵恫瀵笿2鐨勫奖鍝嶃€傘€愮粨鏋溿€戠敱LC/GC-MS鍒嗘瀽鐣寗鏍圭郴鍒嗘硨鐗╁彂鐜板姞宸村柗涓佹槸涓€绉嶆鐗╂簮鐨勫寲瀛︾墿璐?瓒嬪寲鎬ц瘯楠岃〃鏄?鍦ㄦ祿搴︿负1 mM鐨勫姞宸村柗涓佹憾娑插J2鏈夋帓鏂ヤ綔鐢?姝讳骸鐜囪瘯楠屽彂鐜?褰撴祿搴︿负1 mM鏃?J2鐨勬浜$巼杈惧埌93%,褰撴祿搴︾█閲婅嚦0.01 mM鏃?瀵逛簬J2鐨勬浜$巼杈惧埌70%浠ヤ笂銆傘€愮粨璁恒€戞鐗╂簮鐨勫姞宸村柗涓佹憾娑插湪1 mM娴撳害鏃跺J2鍦ㄤ綋澶栨湁鏄庢樉鐨勬姂鍒朵綔鐢?鏈夋垚涓烘柊鍨嬫潃绾垮墏鐨勫彲鑳姐€?/span>

宸村柗涓佹憾娑叉姂鍒舵柟鏍圭粨绾胯櫕浜岄緞骞艰櫕鐨勬渶浣虫祿搴? src=

銆€銆€鍏抽敭璇嶏細銆€鍗楁柟鏍圭粨绾胯櫕; 鐣寗; 鏍圭郴鍒嗘硨鐗? 鍔犲反鍠蜂竵;

銆€銆€Abstract锛氥€€銆怬bjective銆慣o investigate the inhibitory effect of gabapentin solution in tomato root exudates on the second-stage juvenile(J2) of Meloidogyne incognita.銆怣ethods銆慣he components of tomato root exudates were analyzed by LC/GC-MS; the effects of different concentrations of gabapentin solution on the second-stage juvenile(J2) of Meloidogyne incognita were investigated by chemotaxis experiments and mortality experiments.銆怰esults銆慉nalysis of tomato root exudates by LC/GC-MS revealed that gabapentin was a plant-based chemical; Chemotaxis experiments showed that the gabapentin solution at a concentration of 1 mM had a repellent effect on J2 of Meloidogyne incognita; The mortality test found that when the concentration was 1 mM, the mortality of J2 reached 93%, and when the concentration was diluted to 0.01 mM, the mortality for J2 reached more than 70%.銆怌onclusion銆慣he plant-derived gabapentin solution has a significant inhibitory effect on J2 of Meloidogyne incognita in vitro at a concentration of 1 mM, and it may become a new nematicide.

銆€銆€Keyword锛氥€€Meloidogyne incognita; tomato; root exudates; gabapentin;

銆€銆€鏍圭粨绾胯櫕鐥呮槸璁炬柦鐣寗鐢熶骇涓竴绉嶅父瑙佺殑鍦熶紶鐥呭,鍙楃幇瀹炴潯浠跺奖鍝?闃叉不闅惧害楂?杩欏鑷磋鏂戒腑鐣寗鏍圭粨绾胯櫕鐥呮棩鐩婂姞閲?鐥呭涓ラ噸鏃剁敋鑷冲鑷寸粷鏀禰1,2]銆傛潃绾垮墏鏄幇闃舵闃叉不鏍圭粨绾胯櫕鐨勬湁鏁堟墜娈典箣涓€,涓昏鍖呮嫭鍏嬬櫨濞併€佺伃绾跨7銆佹稌鐏▉绛?浣嗘槸杩欎簺鑽墏瀵逛汉鍜岀幆澧冮€犳垚涓€瀹氫激瀹炽€傞殢鐫€涓浗鐜繚鎰忚瘑鐨勪笉鏂彁楂?娉曞緥瑙勫埗搴﹂€愭笎鍋ュ叏,缁胯壊銆佸畨鍏ㄧ殑鏉€绾垮墏瓒婃潵瓒婅浜轰滑鍏虫敞鍜岄潚鐫愩€?/span>

銆€銆€杩戜簺骞存潵,鍏充簬妞嶇墿婧愮殑鏉€绾垮墏姝e湪鍙楀埌浜轰滑鐨勮拷鎹?鍏充簬妞嶇墿鏍圭郴鍒嗘硨鐗╁鎶垫姉鏍圭粨绾胯櫕鎶ラ亾涔熻秺鏉ヨ秺澶氥€傛潹鍥芥爧绛夌殑鐮旂┒鍙戠幇,鎶楁€х暘鑼勭殑鏍圭郴鍒嗘硨鐗╁RKN鎺掓枼浣滅敤,鎰熸€х暘鑼勬牴绯诲垎娉岀墿鍒欏瀵筊KN鍚稿紩浣滅敤[3]銆傜帇鑹宠壋绛夊湪鐮旂┒榛勭摐鏍圭郴鍒嗘硨鐗╂椂鍙戠幇楂樻姉鐮ф湪鐨勬牴绯诲垎娉岀墿鍙互鎶戝埗绾胯櫕鐨勫嵉瀛靛寲[4]銆侱egenhardt绛夌爺绌跺彂鐜板湪鐜夌背鐨勬牴绯诲垎娉岀墿涓壌瀹氬嚭(E)-β-鐭崇鐑兘澶熷惛寮曟牴缁撶嚎铏玔5]銆侶ooks绛夌爺绌跺彂鐜拌強绉戞鐗╂牴绯诲垎娉岀墿涓殑β-涓夎仈鍣诲惄鍙互鎺掓枼鏍圭粨绾胯櫕[6]銆?/span>

銆€銆€缁忚繃鍓嶆湡鐨勬牴绯诲垎娉岀墿浠h阿缁勫垎鏋?鍙戠幇鐣寗鏍郴鏍圭郴鍒嗘硨鐗╀腑鍚湁涓€绉嶅彨鍔犲反鍠蜂竵鐨勭墿璐?骞朵笖璇ュ寲瀛︾墿璐ㄥ彲閫氳繃鍟嗕笟鎵嬫璐緱銆備笉鍚屾祿搴︽搴︾殑鍔犲反鍠蜂竵婧舵恫瀵瑰崡鏂规牴缁撶嚎铏簩榫勫辜铏?J2)杩涜瓒嬪寲鎬у拰姝讳骸鐜囪瘯楠?浣撳绛涢€夊嚭瀵笿2鏈夋姂鍒朵綔鐢ㄧ殑娴撳害,浠ユ鏉ユ帹娴嬪叾杩愮敤浜庣敓浜х殑鍙鎬с€?/span>

銆€銆€1銆?鏉愭枡涓庢柟娉?/span>

銆€銆€1.1銆?妞嶇墿鏉愭枡

銆€銆€鐣寗鍝佺涓篊M妞嶆牚,鐣寗鏍郴鍘熷绉嶅瓙鐢变腑鍥界瀛﹂櫌閬椾紶涓庡彂鑲茬敓鐗╁鐮旂┒鎵€(鍖椾含)鏉庝紶鍙嬬爺绌跺憳鎯犺禒銆?/span>

銆€銆€1.2銆?鏂?娉?/span>

銆€銆€1.2.1銆?鐣寗骞艰嫍鐨勫煿鍏讳笌绠$悊

銆€銆€閲囩敤娓╂堡娴哥娉曚娇绉嶅瓙钀屽彂,寰呭ぇ閮ㄥ垎绉嶅瓙“闇茬櫧”鍚庡湪绌寸洏涓挱绉?鑲茶嫍瀹や腑鍩瑰吇銆傝偛鑻楀鐨勬俯鍏夎缃负:鏄煎娓╁害涓?6 鈩?18 鈩?鍏夌収鏈熶负16 h,鏆楁湡涓? h銆傛牴鎹暘鑼勫辜鑻楃殑鐢熼暱鐘跺喌,閫傛椂琛ュ厖钀ュ吇娑?鍙婃椂娴囨按閫氶銆?/span>

銆€銆€1.2.2 銆佸崡鏂规牴缁撶嚎铏殑绻佹畺涓庣鐞?/span>

銆€銆€鏍圭粨绾胯櫕涓哄崡鏂规牴缁撶嚎铏?Meloidogyne incongnita),鐢卞啘涓氬簲鐢ㄦ柊鎶€鏈寳浜競閲嶇偣瀹為獙瀹よ嚜绻併€傚皢瀹為獙瀹ゆ槗鎰熺暘鑼勬鏍牴绯讳笂娣¢粍鑹插嵉鍧楁寫涓嬪悗钂搁姘存竻娲楀共鍑€骞舵秷姣?鏀剧疆鍦?6 鈩冩亽娓╁煿鍏荤涓煿鑲?24锝?2 h鍐呬笉鏂瀵?鎸戦€夋椿鍔涘己鐨凧2鐢ㄤ簬瓒嬪寲鎬у拰鑷存鐜囪瘯楠屻€?/span>

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銆€銆€閲囩敤娴告牴娉曟敹闆嗙暘鑼勬牴绯诲垎娉岀墿,鐢℉erz绛夊紑鍙戠殑鏂规硶鏀堕泦鏍圭郴鍒嗘硨鐗7]銆傚皢瀹屾暣鐨勬牴鏀惧湪瑁呮湁150 mL钂搁姘村苟涓旂敤閿$當绾稿寘瑁圭殑鐑ф澂涓€傛瘡4 h鏇存崲1娆℃按,鏀堕泦1娆°€備负浜嗚繘涓€姝ュ垎鏋?灏嗘笚鍑烘恫杩囨护骞舵祿缂╄嚦5 mL銆傚緟鐣寗妞嶆牚闀胯嚦4鍙?蹇冩椂杩涜鍒嗘硨鐗╂敹闆嗚瘯楠?姣忓鐞?鏍噸澶嶃€傛敹闆嗙殑鍒嗘硨鐗╃粡杩囪繃婊ゃ€佹祿缂╁悗閲囩敤LC-MS涓嶨C-MS鎶€鏈壌瀹氥€佸垎鏋愭牴绯诲垎娉岀墿鐨勬垚鍒嗙粍鎴?鍖椾含鍑℃槦鍗氬ゥ鐢熺墿鎶€鏈湁闄愬叕鍙告祴瀹?銆?/span>

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銆€銆€灏嗙洿寰勪负5 cm鐨勫煿鍏荤毧搴曢儴閫氳繃涓や釜鍐呭鐜垝鍒嗘垚16閮ㄥ垎銆傚厛鍦ㄥ煿鍏荤毧涓€掑叆0.8 %鐞艰剛绯栫害5 mL,寰呭嚌鍥哄悗澶囩敤銆傚湪RE鍖哄煙鍐呭姞鍏?0 μL寰呮祴鐨勬牴绯诲垎娉岀墿鎴栧姞宸村柗涓佹憾娑?灏?0 μL鍐呭惈鏈夌害30澶碕2鎮诞娑叉帴绉嶄簬涓績鐜疦鍖哄煙,鍦?6 鈩冩亽娓╁煿鍏荤涓粦鏆楁斁缃?4 h銆傚湪鏄惧井闀滀笅鍒嗗埆缁熻1锝?鍖哄強涓績N鍖哄煙鐨勬牴缁撶嚎铏暟閲廩8]銆?/span>

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銆€銆€鍦?2瀛旀澘涓婂涓嶅悓娴撳害(1銆?.1銆?.01 mM)鐨勫姞宸村柗涓佹憾娑茶繘琛孞2姝讳骸鐜囪瘯楠?姣忕娴撳害璇曢獙6娆°€傝嚦灏戣繘琛屼簡3娆$嫭绔嬭瘯楠孾8]銆傚鐓х粍鐢变箼閱囧拰钂搁姘寸粍鎴愩€傚湪鍔犲叆涓嶅悓娴撳害鐨勫姞宸村柗涓佷箣鍓?姣忎釜瀛斿姞鍏?0 μL鍚湁30锝?0澶?J2s鐨勬恫婊淬€傚湪26 鈩冨煿鍏荤榛戞殫鍩瑰吇24 h鍚?鐢?0鍊嶆樉寰暅璁℃暟涓嶆椿鍔ㄧ嚎铏暟閲忕敤浜庤绠楁浜$巼銆?/span>

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銆€銆€2銆?缁撴灉涓庡垎鏋?/span>

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銆€銆€閫氳繃瀵规敹闆嗙殑鐣寗鏍圭郴鍒嗘硨鐗╄繘琛屼唬璋㈢粍瀛﹀垎鏋愬彂鐜?鍦ㄦ牴绯诲垎娉岀墿涓惈鏈夊绉嶆垚鍒嗐€傛牴绯诲垎娉岀墿鐨勪富瑕佹垚鍒嗕竴鍏辨娴嬪埌2 000澶氱鐗╄川,鍏朵腑涓昏鍖呭惈姘ㄥ熀閰?鑲藉強鍏剁被浼肩墿,鑻啔绫?鑻厷绫?鑻敳閰稿強鍏惰鐢熺墿,β-缇熷熀閰稿強鍏惰鐢熺墿,鑳嗘眮閱囥€侀吀鍙婂叾琛嶇敓鐗?纰虫按鍖栧悎鐗╁強鍏剁粨鍚堢墿绛夈€傚杩欎簺鐗╄川杩涜绛涢€夊悗灏嗗彲璐緱鐨勭墿璐ㄨ繘琛屽悗缁瘯楠?琛?涓虹暘鑼勫垎娉岀墿鎴愬垎閮ㄥ垎)銆傚叾涓?鍦ㄦ牴绯诲垎娉岀墿涓姞宸村柗涓佹槸瀛樺湪鍏朵腑鐨?鍏剁浉瀵瑰惈閲忎负80.626 2銆傚姞宸村柗涓佸湪鏍圭郴鍒嗘硨鐗╀腑骞朵笉鏄惈閲忔渶澶氱殑鐗╄川,鐩稿鍏朵粬鐗╄川鏉ヨ鍏跺惈閲忓緢灏?鍏舵墍璧风殑浣滅敤鍙兘鍜屽叾鍚噺鍏崇郴涓嶅ぇ銆傝鐗╄川鐨勬牳璐ㄦ瘮涓?72.131,CAS鍙蜂负60142-96-3,瀹ゆ俯涓嬪憟鐧借壊绮夋湯鑷崇伆鐧借壊缁撴櫠绮夋湯,鍏剁悊鍖栨€ц川绋冲畾銆?/span>

銆€銆€琛? 鐣寗鍒嗘硨鐗╀唬璋㈢粍閮ㄥ垎淇℃伅
琛? 鐣寗鍒嗘硨鐗╀唬璋㈢粍閮ㄥ垎淇℃伅

銆€銆€2.2銆?瓒嬪寲鎬х粨鏋滃垎鏋?/span>

銆€銆€濡傝〃2鎵€绀?閫氳繃瀵瑰崡鏂规牴缁撶嚎铏簩榫勫辜铏?J2)杩涜瓒嬪寲鎬ц瘯楠屽彂鐜?褰揋abapentin婧舵恫鐨勬祿搴︿负1 mM鏃?瀵笿2鏈夋槑鏄剧殑鎺掓枼浣滅敤;褰撴祿搴︾█閲婅嚦0.1銆?.01 mM鏃?Gabapentin瀵笿2鏃犳槑鏄惧惛寮曟垨鏄帓鏂ョ幇璞?瀵圭収琛ㄧ幇鍑虹浉鍚岀幇璞°€?/span>

銆€銆€琛? 鏍圭郴鍒嗘硨鐗╁強涓嶅悓娴撳害鍔犲反鍠蜂竵瀵笿2鐨勮秼鍖栨€?br /> 琛? 鏍圭郴鍒嗘硨鐗╁強涓嶅悓娴撳害鍔犲反鍠蜂竵瀵笿2鐨勮秼鍖栨€? src=

銆€銆€娉?Cf<0.5鎺掓枼,Cf>2鍚稿紩,0.5≤Cf≤2鏃犲奖鍝嶃€傝〃涓暟鎹负骞冲潎鍊?plusmn;鏍囧噯宸?涓嶅悓瀛楁瘝琛ㄧず宸紓鏋佹樉鐫€(P<0.01)

銆€銆€Note: Cf<0.5 repels, Cf>2 attracts, 0.5≤Cf≤2 neutral. The data in the table are mean±standard deviation. Different letters indicate that the difference is extremely significant (P<0.01)

銆€銆€2.3銆?J2姝讳骸鐜囩粨鏋滃垎鏋?/span>

銆€銆€濡傚浘1鎵€绀?閫氳繃瀵笿2杩涜姝讳骸鐜囪瘯楠屾椂鍙戠幇,褰撴祿搴︿负1 mM鍜?.1 mM鏃?24 h绾胯櫕姝讳骸鐜囧垎鍒揪鍒?3%銆?5%;褰撴祿搴︿负0.01 mM鍜?.001 mM鏃?24 h绾胯櫕姝讳骸鐜囧垎鍒负72%銆?5%;褰撴祿搴︾█閲婂埌0.000 1 mM鏃舵浜$巼涓嬮檷鍒?6%,铏界劧涓庡鐓х浉姣斾粛鏈夋樉鐫€宸紓,浣嗘槸鐢变簬姝讳骸鐜囧凡缁忎綆浜?0%,鑷存浣滅敤寮便€?/span>

銆€銆€鍥? 涓嶅悓娴撳害鍔犲反鍠蜂竵瀵笿2鐨勮嚧姝荤巼姣旇緝(P<0.01)
鍥? 涓嶅悓娴撳害鍔犲反鍠蜂竵瀵笿2鐨勮嚧姝荤巼姣旇緝(P<0.01)

銆€銆€Fig.1 Comparison of lethality of gabapentin to J2 at different concentrations (P<0.01)

銆€銆€缁间笂鎵€杩?閫氳繃LC/GC-MS鍙戠幇鍦ㄧ暘鑼勬牴绯诲垎娉岀墿涓惈鏈変竴绉嶅悕涓哄姞宸村柗涓佺殑鍖栧鐗╄川,璇ョ墿璐ㄥ娓╀笅鍖栧鎬ц川绋冲畾;閫氳繃J2浣撳瓒嬪寲鎬у拰姝讳骸鐜囪瘯楠屽彂鐜?褰撳姞宸村柗涓佹憾娑叉祿搴︿负1 mM鏃?瀵笿2鏈夋槑鏄剧殑鎺掓枼浣滅敤骞朵笖J2姝讳骸鐜囬珮杈?2 %;褰撴祿搴︿负0.1锝?.001 mM鏃?鍔犲反鍠蜂竵婧舵恫瀵笿2鏃犳槑鏄炬帓鏂ョ幇璞?浣咼2鐨勬浜$巼渚濇棫瓒呰繃70%銆傝繖璇存槑鍔犲反鍠蜂竵婧舵恫鍦ㄦ祿搴︿负1 mM鏃跺鍗楁柟鏍圭粨绾胯櫕浜岄緞骞艰櫕(J2)鏈夋槑鏄剧殑鎶戝埗浣滅敤銆?/span>

銆€銆€3銆佽 璁?/span>

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銆€銆€[1] 娈电帀鐜?妞嶇墿绾胯櫕瀛M].鍖椾含:绉戝鍑虹増绀?2011
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銆€銆€鍘熸枃鍑哄锛氭潹杩涘北,璧垫枃瓒?渚挵棰?鏉庤悓,寮犵惔鏋?鐜嬬粛杈?妞嶇墿婧愮墿璐ㄥ姞宸村柗涓佸鍗楁柟鏍圭粨绾胯櫕鐨勬姂鍒朵綔鐢╗J].鍖椾含鍐滃闄㈠鎶?2020,35(04):31-34.

 

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